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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2015 Apr 3;194(10):4705–4716. doi: 10.4049/jimmunol.1402979

Figure 4.

Figure 4

Epoxygenase-derived EETs regulate fibronectin levels in macrophages. (A) Measurement of Fn1 expression levels by qRT-PCR in WT (basal), Cyp2j4−/− BMDMs (basal and following incubation with different EETs, 1μM). *, P < 0.01 compared to WT. (B) Human monocyte-derived macrophages (MDMs) from different healthy donors were incubated with 11,12 EETs (1μM) and fibronectin levels were measured by Western Blot (upper panel; donor 1 and donor 3) and qRT-PCR (lower panel; all donors). PPARγ protein levels were reduced when human MDMs from different donors were incubated with 11,12 EETs (1μM, right panel). (C) siRNA knock-down of human CYP2S1 and the expression levels of CYP2S1 (left panel) and FN1 (right panel). Control samples were incubated with non-targeting scrambled siRNA. FN1 expression was measured in control and siRNA conditions, with or without addition of 11,12 EETs (1μM).