Extended Data Figure 6. Sensitivity of POLR2A^loss cells to POLR2A inhibition is independent of p53.

a, Schematic illustration of the Cas9/sgRNA-targeting sites in the TP53 gene. Two sgRNA-targeting sequences are shown and the PAM sequences are highlighted in red. b, Efficiency of the Cas9-mediated cleavage of TP53 in HCT116 cells measured by Surveyor assay. c, Protein levels of POLR2A and p53 in a panel of isogenic HCT116 cells. d, Growth curves of POLR2A^neutral and POLR2A^loss xhCRC cells. e, Growth curves of POLR2A^neutral and POLR2A^loss HCT116 cells. f, g, Crystal staining images (f) and cell survival curves (g) of POLR2A^neutral and POLR2A^loss HCT116 cells treated with α-Amanitin. h, Cell survival curves of POLR2A^neutral and POLR2A^loss HCT116 cells in response to the treatment of Ama-HEA125. Data are mean and s.d. of three independent experiments in the figure.