Figure 2. Time course for the subcellular localization of myc-tagged myosin10/11/14/9-tagged myosin regulatory light chain (MRLC) and Venus-tagged myosin regulatory light chain (MRLC9/12-Venus) at the stages indicated.
Arrows in A indicate contacts of cells with the basement membrane, and arrowheads in C indicate anteriorly localized protein. Densitometry readings across the center of four cells demonstrate the transition in MRLC9/12 localization from stage 21 to stage 22 (charts in B and C). (D) At stage 24, the anterior localization of both proteins persists. Anterior is to the right in all panels. Scale bars are 10 μm.