Figure 5. MRLC9/12 localization is resistant to cytochalasin B and blebbistatin.
(A) Control, vehicle-treated embryo shows polarization of MRLC9/12 to the anterior membrane of notochord cells. (B and C) MRLC9/12 polarization is resistant to cytochalasin B and latrunculin B treatments. MRLC9/12-Venus is labeled green, and H2A-RFP (nuclei) is labeled red. (D) Percentage of notochord cells showing polarized MRLC9/12 for three treatment regimes. The number of cells scored is indicated at the bottom of the columns. e. st., early stage; l. st., late stage. (E and F) Persistence of MRLC9/12-Venus polarization (white) in embryos treated with blebbistatin or vehicle (DMSO) from stage 21 to stage 23. Arrowheads indicate anteriorly localized proteins.