Figure 6. Recovery of polarity following cytochalasin B treatment.
(A) Time lapse of nuclear repolarization following removal of embryos from cytochalasin B. Times are from end of cytochalasin B treatment. (B) Recovery of Pk-myc polarization following cytochalasin B treatment and wash out. Anterior is to the right in all panels. (C and D) Blebbistatin blocks recovery from cytochalsin B treatment. Following removal of embryos from cytochalsin B, embryos were either treated with vehicle (DMSO) (C) or 10 μm blebbistatin (D). Arrowheads indicate anterior localized Pk. Scale bar in A is 5 μm.