Figure 7. Notochord A/P polarity does not require microtubular network.
(A) Visualization of the notochord microtubule network with GFP-tagged ensconsin, and B, disruption of the network with 10 μm nocodazole. (C–E) Nocodazole treatment from stage 21 disrupts neither nuclear (D) nor Pk-myc (E) polarity. Embryos/cells are shown at stage 23. Similarly, nocodazole treatment at stage 21 does not disrupt MRLC9/12 polarity (control, F; treated, G). (H) Cell nuclei properly repolarized in the presence of nocodazole following cytochalasin treatment. Arrowheads indicate anterior localized MRLC9/12. Scale bars are 10 μm.