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Comparison of the enrichment efficiency among PolyMAC-Ti, PolyMAC-Fe and IMAC, including A) number of unique phosphopeptides identified and B) percent enrichment selectivity (# unique phosphopeptides/total # of peptides). C) Overlap of the unique phosphopeptides identified by PolyMAC-Ti and PolyMAC-Fe. A 50 μg lysate from pervanadate-treated DG-75 cells was used for the comparison; error bars represent 3 separate enrichment experiments.
