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. 2015 Apr 9;7(4):1235–1252. doi: 10.3390/toxins7041235

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical localization of CPB (Clostridium perfringens beta-toxin) in porcine jejunal cryosections. Cryosections of porcine neonatal jejunum were incubated with C. perfringens type C NCTC 3180 supernatant (diluted 1:10 in PBS). CPB was subsequently detected by immunohistochemistry on endothelial cells (arrowheads) in the lamina propria (A) and submucosa (B), but not on epithelial cells (arrows). (C) Magnification of B depicting endothelial CPB signal. Representative pictures from one out of two independent experiments, magnification 400×.