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. 2015 Apr 9;7(4):1235–1252. doi: 10.3390/toxins7041235

Figure 2.

Figure 2

Figure 2

Immunohistochemical localization of CPB in the subepithelial lamina propria of porcine jejunal explants. (A) Jejunal explants were either unmodified (unmodified), the epithelium was mechanically damaged (damaged epithelium), or the tunica serosa and tunica muscularis were mechanically detached (detached mucosa). Explants were incubated with C. perfringens type C supernatant (JF 3721 diluted 1:10 in RPMI), fixed, and CPB was detected by immunohistochemistry. After 2 h of incubation, mild to moderate CPB signals were detected at endothelial cells close to the damaged epithelium whereas undamaged explants showed no or only very mild signals at 2 h. After 5 h of incubation CPB signals were overall stronger in tissue with a mechanically damaged epithelium. Binding of CPB to the epithelium was not detectable in any explant. Representative pictures from one out of three independent experiments, magnification 400×. (B) Mean CPB signal scores and standard deviation at endothelium-villi of eight explants in three independent time course experiments. (C) Mean CPB signal scores and standard deviation at endothelium-submucosa (fotographs depicted in Figure S1) of eight explants in three independent time course experiments.