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. 2015 Apr 9;7(4):1235–1252. doi: 10.3390/toxins7041235

Figure 4.

Figure 4

Reduction of TEER upon basolateral exposure of IPEC-J2 cells to C. perfringens type C culture supernatants but not rCPB. IPEC-J2 cells were incubated in the basolateral compartment of the Transwell® with the same toxins and supernatants (diluted 1:10 and 1:5) as in Figure 3. Aerolysin and the C. perfringens type C supernatant NCTC 3180 (1:10) caused a rapid and sustained drop in TEER. This effect could not be inhibited by neutralization of CPB using mAb-CPB. Supernatants of JF 3721 induced a drop in TEER within 12 h and supernatants of JF 3693 within 48 h. rCPB (32 µg/mL), or TGY (1:5) resulted in a small drop of TEER values, which however never dropped below 2 kΩ cm2 and subsequently increased towards the end of the experiment. Values represent means of three independent experiments with a total of eight separate Transwells®. Error bars represent two-fold standard deviation. Asterisks indicate values which differ significantly from the TGY control group.