Table 4.
Nucleotide sequence of primers for real-time PCR.
| Gene | Primer sequence | Amplicon | Ensembl access | References | |
|---|---|---|---|---|---|
| Size | Intron 1 | ||||
| Housekeeping genes | |||||
| GAPDH | F (300 nM) TCCTAGGATACACAGAGGACCA R (300 nM) CGGTTGCTATATCCAAACTCA |
151 bp | 2(499) | ENSGALG00000014442 | Present study |
| RPL4 | F (300 nM) TTATGCCATCTGTTCTGCC R (300 nM) GCGATTCCTCATCTTACCCT |
235 bp | 2(893) | ENSGALG00000007711 | Present study |
| Pro-inflammatory cytokines | |||||
| IL-1β | F (300 nM) GCATCAAGGGCTACAAGCTC R (300 nM) CAGGCGGTAGAAGATGAAGC |
131 bp | 1(87) | ENSGALG00000000534 | [48] |
| IL-6 | F (300 nM) GAATGTTTTAGTTCGGGCACA R (300 nM) TTCCTAGAAGGAAATGAGAATGC |
130 bp | 0 | ENSGALG00000010915 | Present study |
| IL-8 | F (300 nM) GCGGCCCCCACTGCAAGAAT R (300 nM) TCACAGTGGTGCATCAGAATTGAGC |
146 bp | 2(1210) | ENSGALG00000011670 | Present study |
| Treg signature | |||||
| IL-10 | F (300 nM) GCTGAGGGTGAAGTTTGAGG R (300 nM) AGACTGGCAGCCAAAGGTC |
121 bp | 2(1127) | ENSGALG00000000892 | Present study |
| TGFβ1 | F (300 nM) CGGGACGGATGAGAAGAAC R (300 nM) CGGCCCACGTAGTAAATGAT |
258 bp | no data | [49] | |
| SOCS1 | F (300 nM) CAAGCGGATTTCAGTAGCATC R (300 nM) GGCTCAGACTTCAGCTTCTCA |
110 bp | no intron | ENSGALG00000007158 | Present study |
| Th17 & Th1 signature | |||||
| IL-17 | F (300 nM) TATCAGCAAACGCTCACTGG R (300 nM) AGTTCACGCACCTGGAATG |
110 bp | 1(666) | ENSGALG00000016678 | Present study |
| IL-21 | F (300 nM) GCTTTCAAAGACAATTGACCATC R (300 nM) TACAGCTGTGAGCAGGCATC |
106 bp | 2(3765) | ENSGALG00000011844 | Present study |
| IFN-γ | F (300 nM) AGCTGACGGTGGACCTATTATT R (300 nM) GGCTTTGCGCTGGATTC |
259 bp | 2(998) | ENSGALG00000009903 | [49] |
1 number of introns spanned in the design of primers, the brackets report the total size of introns (in bp). GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; IFN-γ, Interferon-γ; IL, Interleukin; RPL4, Ribosomal protein L4; SOCS1, Suppressor of cytokine signaling 1; TGFβ1, Transforming growth factor β 1.