Table 3.

List of Novel miRNAs expressed and regulated across mosquito developmental stages

S. No	Length	Mature miRNA sequence	Pre-miRNA sequence	Novel miRNA	Tags Per Million (TPM)
					LM	LF	PM	PF	AM	AF
1	20	AGTCATTAATGATATTAGAC	AUUUAUGGGAAAUAAUCUCUUUAAUCAGGCUUUAUAGUCAUUAAUGAUAUUAGACUGCAAUUCUAAAGGA AUAAUUUAAUUUAUUAAGGCUUAAGAAUUAAAAGAUUAAUACAAAUAUUUUCA	ast-Novel-1-3p	0.37	0.00	0.06	0.05	0.08	0.00
2	21	ATAATTTAATTTATTAAGGCT	AUUUAUGGGAAAUAAUCUCUUUAAUCAGGCUUUAUAGUCAUUAAUGAUAUUAGACUGCAAUUCUAAAGGA AUAAUUUAAUUUAUUAAGGCUUAAGAAUUAAAAGAUUAAUACAAAUAUUUUCA	ast-Novel-1-5p			0.18	0.16	2.84	0.18
3	23	GTATTCAATTTGTATATCGTCGT	GUUGGACCUUACUUAAAUUUGUAUUCAAUUUGUAUAUCGUCGUCAUCAGAAUAUAUUAUAAGAUUAAUAAUUUUCUAAAUAUUUUAUUAAAUAAUAUGUCAGGUCAAGGUGCAGUUUAUGUUUAAGUAG	ast-Novel-2-3p			0.18	0.10	0.08
4	19	ATTGATAATCCACGTTGGA	GUUGGACCUUACUUAAAUUUGUAUUCAAUUUGUAUAUCGUCGUCAUCAGAAUAUAUUAUAAGAUUAAUAAUUUUCUAAAUAUUUUAUUAAAUAAUAUGUCAGGUCAAGGUGCAGUUUAUGUUUAAGUAG	ast-Novel-2-5p	0.37		0.54	0.16	0.11
5	24	TTAAGATTGGTATGATTAGCGTCT	UUUCCUUUAAGAUUGGUAUGAUUAGCGUCUUGUUUAGCAGAAACUAAUCGUACUCCUUUU	ast-Novel-3-3p				0.16	0.12	0.24
6	24	AAACTAATCGTACTCCTTTTGATT	UUUCCUUUAAGAUUGGUAUGAUUAGCGUCUUGUUUAGCAGAAACUAAUCGUACUCCUUUU	ast-Novel-3-5p					0.16
7	18	AATGGGTAGTCGAAGATT	AAUUGCUCAUGGUUUAUGUUCAUCUGGAUUAUUUUGUUUAGCUAAUAUUUCUUACGAACGAAUGGGUAGUCGAAGAUUAUUAAUUAAUAA	ast-Novel-4-3p			6.00	0.47	0.16
8	22	AATTGCTCATGGTTTATGTTCA	AAUUGCUCAUGGUUUAUGUUCAUCUGGAUUAUUUUGUUUAGCUAAUAUUUCUUACGAACGAAUGGGUAGUCGAAGAUUAUUAAUUAAUAA	ast-Novel-4-5p					0.3
9	21	CGTAGTTTCTACATTAGGAGT	UUUUACUUUUUUUGUAGGAUCAAUAUGAUUUAUACCCGUAGUUUCUACAUUAGGAGUAAU	ast-Novel-5-3p			0.12	0.10	0.04
10	21	ACTTTTTTTGTAGGATCAATA	UUUUACUUUUUUUGUAGGAUCAAUAUGAUUUAUACCCGUAGUUUCUACAUUAGGAGUAAU	ast-Novel-5-5p			0.60	0.42	0.56	0.18
11	25	TTTCGGATATGAATCAAAGTAATTT	ACCACUUAAAUUUCGGAUAUGAAUCAAAGUAAUUUUCAUCAAUUCCCUCUACCCUCAUGAGCGAGUGAUGUAAUGUACUCUCGUUCUGGACGAUUUUCAACCACUUAAAU	ast-Novel-6-3p	100.76	44.78	95.03	141.73	135.20	796.74
12	21	CGTTCTGGACGATTTTCAACC	ACCACUUAAAUUUCGGAUAUGAAUCAAAGUAAUUUUCAUCAAUUCCCUCUACCCUCAUGAGCGAGUGAUGUAAUGUACUCUCGUUCUGGACGAUUUUCAACCACUUAAAU	ast-Novel-6-5p	0.37				0.6
13	23	AGCCGAACCAGCAGTACGAGTTT	GACCCGACCGAGCCGAACCAGCAGUACGAGUUUUAACGCACGUUUCUUCGACUGGGAGCGUGGCGUCUCCUGUAACGCGGCUACUCGUGG	ast-Novel-7-3p					0.04
14	22	CGTCTCCTGTAACGCGGCTACT	GACCCGACCGAGCCGAACCAGCAGUACGAGUUUUAACGCACGUUUCUUCGACUGGGAGCGUGGCGUCUCCUGUAACGCGGCUACUCGUGG	ast-Novel-7-5p		0.28	0.30	0.31	0.04	0.18