Table 4.
List of enriched pathways functional during different stages of mosquito development
| (I) | miRNAs differentially expressed in male and female adult mosquitoes | |
| Down-regulated miRNAs in females: miR-2c, miR-285, miR-219, miR-7 and miR-2765 | ||
| Up-regulated miRNAs in females: miR-989 | ||
| Significant clusters identified | Enrichment score | |
| 1 | Cellular polysaccharide biosynthetic process | 2.1 |
| 2 | Chaperone | 1.8 |
| 3 | Hexose metabolic pathway | 1.3 |
| 4 | Heme binding | 1.1 |
| 5 | Phagocytotic pathways | 1.09 |
| (II) | miRNAs regulated from larval to pupa transition of male mosquito | |
| Down-regulated miRNAs in pupa male: miR-2c, miR-375, miR-7, miR-1175, miR-263a, miR-1174 and miR-34 | ||
| Up-regulated miRNAs in pupa male: miR-100, miR-193, miR-1-3p, miR-1890, miR-277, miR-989 and miR-285 | ||
| Significant clusters identified | Enrichment score | |
| 1 | phagocytosis and membrane organization | 2.6 |
| 2 | polysaccharide metabolic process | 2.1 |
| 3 | protease activity | 1.9 |
| 4 | peptidase activity | 1.6 |
| 5 | nuclear transport | 1.1 |
| (III) | miRNAs regulated from transition of pupa to adult male mosquito | |
| Down-regulated miRNAs in adult male: miR-2765, miR-193, miR-282, miR-989, miR-2a-5p, miR-9a, miR-1890 and miR-965 | ||
| Up-regulated miRNAs in adult male: miR-10-5p, miR-219, miR-2c, miR-210-3p, miR-190-3p, miR-34 and miR-1891 | ||
| Significant clusters identified | Enrichment score | |
| 1 | gelsolin –actin binding protein | 2.2 |
| 2 | polysaccharide biosynthetic process | 2 |
| 3 | zinc finger | 1.8 |
| 4 | vesicle mediated transport | 1.7 |
| 5 | protein biosynthesis | 1.6 |
| 6 | translation | 1.5 |
| 7 | vitamin and cofactor binding | 1.4 |
| 8 | nucleoside binding | 1.3 |
| 9 | protein catabolic process | 1.2 |
| 10 | insect pheromone/odorant binding protein phBP | 1.1 |
| (IV) | miRNAs regulated from larval to pupa transition of female mosquito | |
| Down-regulated miRNAs in pupa female: miR-7, miR-263a, miR-375, miR-317, miR-34, miR-1174, miR-315 and miR-993-3p | ||
| Up-regulated miRNAs in pupa female : miR-1-3p, miR-let-7, miR-277, miR-133-3p, miR-285, miR-193, miR-998 and miR-184b | ||
| Significant clusters identified | Enrichment score | |
| 1 | endocytosis | 3 |
| 2 | polysaccharide metabolic process | 1.9 |
| 3 | cell-redox homeostasis | 1.7 |
| 4 | peptidase | 1.5 |
| 5 | amino acid metabolic pathway | 1.4 |
| (v) | miRNAs regulated from transition of pupa to adult female mosquito | |
| Down-regulated miRNAs in adult female: miR-2765, miR-2c, miR-1-5p, miR-193, miR-282, miR-2944a-5p, miR-285 and miR-9a | ||
| Up-regulated miRNAs in adult female : miR-927-5p, miR-375, miR-980, miR-989, miR-210-3p, miR-317, miR-34 and miR-1891 | ||
| Significant clusters identified | Enrichment score | |
| 1 | phagocytosis | 2.4 |
| 2 | cellular carbohydrate biosynthetic process | 2.1 |
| 3 | protein biosynthesis | 1.6 |
| 4 | vitamin binding | 1.5 |
| 5 | golgi membrane | 1.1 |
| 6 | ATP binding | 1.1 |
| 7 | hydrolase activity | 1.1 |
| 8 | transcription | 1.1 |
These pathways were identified using NIH DAVID functional annotation clustering tool with E score =0.1.