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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Breast Cancer Res Treat. 2015 Mar 28;150(3):535–545. doi: 10.1007/s10549-015-3354-y

Figure 3. TRβ–mediated resistance involves cAMP/PKA signaling.

Figure 3

A. Genes from the top KEGG pathway, chemokine signaling, in TRβ KD cells compared to EV group were identified using DAVID. B and C. HCC2185 and HCC202 cells (EV and shTRβ) were treated with DOX, a cAMP inhibitor, alone or in combination for 3 days, and MTT growth assays were performed.* p<0.05 and ** p<0.01. D and E. HCC2185 and HCC202 cells were treated with DOX or the PKA inhibitor H89, alone or in combination and growth assessed using MTT assays. F. HCC202 cells were treated with H89 (1 uM) or Dox (200 nM), alone or in combination for 4 days, and analyzed by western blot analysis. TRβ, pPKA, PKA, cleaved caspase 3, caspase 3, cleaved PARP and PARP expression were examined using GAPDH as a loading control. G. Densitometry of western blot shown in panel F.