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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2014 Sep 22;281(2):174–184. doi: 10.1016/j.taap.2014.08.027

Figure 4. Western analysis of cytosolic and mitochondrial fractions 4 h after APAP treatment.

Figure 4

Treatment groups were as described in Figure 1. Liver cytosol and mitochondrial fractions were prepared from liver samples 2 and 4 h post APAP administration. A 100 ug aliquot of protein was loaded in each lane and a 1:1000 primary Anti-4-HNE Michael adduct antibody was used to assess 4-HNE adduction. Representative gels for cytosol (Panel A) and mitochondria (Panel B) are shown for the 4 h post APAP treatment time. Densitometry results are shown in Figure 5.