Table 2.

Proteins identified in 2D gel of Mitochondrial 4 h post APAP treatment using Mass Spectrometry.

Spot	Protein	Accession number	Molecular weight (kDa)	Num. of peptides	% Coverage	Calc. pI
1	Hypoxia up-regulated protein 1	IPI00123342.4	111.1	46	51.2	5.19
2	Pyruvate carboxylase, mitochondrial	IPI00114710.4	129.6	40	38.3	6.71
	Carbamoyl-phosphate synthase	IPI00111908.8	164.5	36	26.7	6.92
3	Sarcosine dehydrogenase	IPI00136213.5	101.6	40	45.6	6.74
	Carbamoyl-phosphate synthase	IPI00111908.8	164.5	49	36.0	6.92
4	GRP78 (glucose-regulated protein)	IPI00319992.1	72.4	24	38.0	5.1
	ATP synthase subunit beta	IPI00468481.2	56.3	13	37.6	5.3
5	Aldehyde dehydrogenase	IPI00111218.1	56.5	6	21.8	7.6
	ATP synthase subunit beta	IPI00468481.2	56.3	8	23.8	5.3
6	Liver carboxylesterase 31	IPI00381178.3	63.3	14	34.9	6.1
	Carbamoyl-phosphate synthase	IPI00111908.8	164.5	35	29.7	6.9
	Acyl-CoA dehydrogenase family member 9	IPI00331710.2	68.7	13	26.2	7.4
7	Protein disulfide-isomerase	IPI00133522.2	57.0	50	70.3	4.8
8	Aldehyde dehydrogenase	IPI00111218.1	56.5	32	55.3	7.6
	Alanine aminotransferase 1	IPI00154045.3	55.1	23	55.9	6.6
9	Aldehyde dehydrogenase, mitochondrial	IPI00111218.1	56.5	33	55.3	7.6
	Alanine aminotransferase 1	IPI00154045.3	55.1	18	50.0	6.6
10	3-ketoacyl-CoA thiolase	IPI00226430.3	41.8	37	85.9	8.1
	Betaine-homocysteine S-methyltransferase 1	IPI00130950.1	45.0	15	52.83	7.9

The 10 spots indicated in Figure 6 were excised, digested, and analyzed via LC/MS-MS. The identified proteins within each spot, their accession number, molecular weight, the number of peptides identified in the MS-MS analysis, the percent protein coverage of the identified peptides and the calculated isoelectric point (pI) are listed in the table.