Fig. 3.
YTR107 inhibits pNPM1 recruitment to chromatin in irradiated cells.
A Representative immunoblot illustrating the extraction of non-chromatin bound pT199NPM1 in 420 mM NaCl buffer from nuclei of A549 cells. Cells were exposed to DMSO or various concentrations of YTR107 for 30 min prior to, during, and for 90 min after 0 or 6 Gy. B Quantification of buffer soluble pNPM1 (± SD) from nuclei of irradiated cells relative to non-irradiated cells, N=3 experiments.