Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Nat Genet. 2014 May 25;46(7):722–725. doi: 10.1038/ng.2986

Figure 3.

Figure 3

SMO Leu412Phe enhances ameloblast-lineage cell proliferation. (a) Overexpression of wild-type SMO, SMO Trp535Leu, SMO Leu412Phe or empty vector control in mouse ameloblast-lineage (ALC) cells, shown by protein blot (antibody to Myc tag). GAPDH serves as a loading control. (b) Relative cell proliferation (in optical density (OD) units) evaluated by WST-1 assay (Roche) 24, 48 and 72 h after plating equal numbers of cells. Overexpression of SMO constructs significantly enhances cell proliferation compared to empty vector control; SMO Leu412Phe also enhances proliferation in comparison to wild-type SMO (two-sided Student’s t test, P values indicated). SMO expression was engineered by lentiviral transduction, and stable cell pools (with approximately 1 × 105 independent integrations) were assayed. Cell proliferation was evaluated by three independent cell platings; error bars, s.d. Results presented are representative of three independent trials.