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. Author manuscript; available in PMC: 2016 Apr 6.
Published in final edited form as: Dev Cell. 2015 Mar 26;33(1):22–35. doi: 10.1016/j.devcel.2015.01.033

Figure 6.

Figure 6

Eya1 promotes Shh signaling in cerebellum. (A) Eya1 is expressed in Purkinje cells and granule cell precursors in the external granule cell layer (EGL). In situ hybridization of Eya1, Gli1 and Ptch1 in E18.5 Eya1+/+ and Eya1−/− cerebella; scale bar = 25 μm. (B) Western blot of Gli1 in Eya1+/+ and Eya1−/− E18.5 cerebellum. (C) Gli1, Ptch1 and Shh mRNA levels in Eya1+/+ and Eya1−/− cerebellum. (D) Gli1 and Gli3 immunoblots of Eya1+/+ and Eya1−/− E18.5 cerebellar lysates. Eya1−/− cerebella have decreased Gli1 protein with no change in Gli3R levels.