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Schematic of the pRH3 cloning vector. Plasmid pRH3 was constructed by marker swap transformation, replacing the URA3+ marker of plasmid pSP2 (Cottarel et al. 1993) with the lys2+ gene. Unique restriction sites in the polylinker are shown in bold. The relative locations of polylinker sites that are present elsewhere is the plasmid are also shown
