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. Author manuscript; available in PMC: 2015 May 4.
Published in final edited form as: Biochem J. 2014 Dec 1;464(2):251–258. doi: 10.1042/BJ20140845

Figure 2. Munc18-1 and Munc18c do not directly interact.

Figure 2

Purified untagged recombinant proteins (2.5 μg of each) were combined and immunoprecipitated (IP) with mouse anti-Munc18-1 (A) or rabbit anti-Munc18c (B) antibodies. Co-immunoprecipitated proteins were resolved by 10% SDS-PAGE for immunoblot detection (IB). IgG was used to control for antibody specificity. Input proteins were taken from each reaction to confirm the presence of each protein in the reaction. Data are representative of at least three independent experiments using two or more independent batches of recombinant proteins.