Fig. 6. Neutrophils lacking functional CXCR4 expression show increased cell survival.
A, Treatment of HL60 cells with AMD3100 inhibits differentiation of HL60 cells into neutrophils as demonstrated by a reduction in the percentage of Ly6GhighCXCR4+ (left panel) and Ly6GhighCXCR4+ cells (right panel) (**P < 0.01 compared to control HL60 cells, ##P < 0.01 compared to RA differentiated HL60 cells). B, AMD3100 reduced neutrophil precursor proliferation during differentiation (**P < 0.01). C, Myeloperoxidase activity (**P < 0.01). D, Percentage of cell survival (***P < 0.001). E, Relative gene expression of Akt in neutrophils differentiated in the presence or absence of AMD3100 (*P < 0.05).