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RhoA decreases the transactivation activity of NFAT. Jurkat T cells were transfected with 5× GAL4-luciferase and plasmids encoding the GAL4-NFAT or GAL4-NF-κB fusion proteins in the presence or absence of RhoA63L. After 48 h, the cells were harvested, and luciferase activity was measured to determine transcriptional activation. Data are shown as percent activity relative to vector from four (GAL4-NFAT) or three (GAL4-NF-κB) independent experiments.
