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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 8;194(10):4615–4619. doi: 10.4049/jimmunol.1402554

FIGURE 1. TCR signal strength regulates PTEN protein levels.

FIGURE 1

(A) PTEN activity in simulations of CD4 T cell differentiation leading to Th (solid line) and Treg fate (dotted line) reported as percentage of simulation trajectories. (B) PTEN protein levels were tracked by Western blotting of activated CD4+ T cells under low- (upper panel) and high- (bottom panel) dose conditions. (C) Actin-normalized PTEN values were plotted. D and E CD4+ T cells were activated under low dose TCR stimulation in the presence (dashed line) or absence (solid line) of a PTEN inhibitor (SF1670). The development of Th (CD25+ Foxp3−) (D) or Treg (CD25+ Foxp3+) (E) was assessed by flow cytometry on gated CD3+ CD4+ T cells. Results in C, D and E represent the mean ± SEM from 3 independent experiments.