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. Author manuscript; available in PMC: 2015 May 4.
Published in final edited form as: Biochim Biophys Acta. 2015 Feb 4;1852(5):882–892. doi: 10.1016/j.bbadis.2015.01.019

Fig. 4.

Fig. 4

Measurement of calpain activation in H9c2 cells following H/R. H9c2 cells were subjected to a 24-hour hypoxia followed by a 24-hour re-oxygenation. (A) Calpain enzymatic activity was measured in H9c2 cells. Data are mean ± SD from 3 different experiments. *P < 0.05. (B) is a representative western blot for a 75 kDa active fragment of calpain-1 and (C) for the cleavage of α-spectrin (140 kDa), a natural substrate of calpain-1.