Table 1. Sequence characteristics of six DNA regions of Schisandraceae (Outgroup taxon excluded).
| ITS1 | ITS2 | ITS | trnH-psbA | matK | rbcL | |
|---|---|---|---|---|---|---|
| Universality of primers | - | - | Yes | Yes | Yes | Yes |
| Percentage PCR success (%) | - | - | 98.19 | 100 | 100 | 99.09 |
| Percentage sequencing success (%) | - | - | 100 | 100 | 100 | 100 |
| No. of species (no. of individuals) | 33 (123) | 33 (123) | 33 (123) | 33 (114) | 33 (114) | 32 (118) |
| No. of singleton species | 1 | 1 | 1 | 8 | 5 | 5 |
| Aligned sequence length (bp) | 299 | 229 | 695 | 579 | 826 | 672 |
| Parsimony-informative sites (bp) | 98 | 67 | 170 | 85 | 60 | 27 |
| Variable sites (bp) | 104 | 77 | 188 | 94 | 65 | 29 |
| No. of indels (length range) | 26 (1–23) | 9 (1–2) | 36 (1–23) | 41 (1–46) | 2 (6) | 0 |
| Average interspecific distance (range) (%) 1 | 15.09 (0–29.47) | 10.75 (0–21.65) | 9.88 (0–19.08) | 6.96 (0–14.12) | 2.84 (0–5.91) | 1.41 (0–2.80) |
| Average intraspecific distance (range) (%) 1 | 0.15 (0–0.51) | 0.28 (0–2.70) | 0.17 (0–1.10) | 0. 09 (0–0.84) | 0.04 (0–0.34) | 0.02 (0–0.11) |
| Average interspecific distance (range) (%) 2 | 3.28 (0–6.98) | 3.16 (0–5.96) | 2.47 (0–4.87) | 2.44 (0–7.05) | 0.86 (0–1.85) | 0.66 (0–1.19) |
| Average intraspecific distance (range) (%) 2 | 0.15 (0–0.51) | 0.10 (0–0.71) | 0.11 (0–0.30) | 0.15 (0–0.84) | 0.06 (0–0.34) | 0.03 (0–0.11) |
| Average interspecific distance (range) (%) 3 | 2.10 (0–4.73) | 1.74 (0–4.05) | 1.47 (0–2.86) | 1.04 (0–2.26) | 0.13 (0–0.49) | 0.13 (0–0.30) |
| Average intraspecific distance (range) (%) 3 | 0.13 (0–0.41) | 0.54 (0–2.70) | 0.26 (0–1.10) | 0.02 (0–0.13) | 0 | 0.01 (0–0.10) |
1The distance based on the data from Schisandraceae.
2The distance based on the data from Schisandra and Kadsura.
3The distance based on the data from Illicium.