Table 4. Identification success rates of single regions and their combinations using TAXONDNA program under ‘best match’ and ‘best close match’ methods based on the data from Schisandraceae.
DNA barcodes | N * species | N sequences | Best match (%) | Best close match (%) | Threshold (%) | |||||
---|---|---|---|---|---|---|---|---|---|---|
Correct | Ambiguous | Incorrect | Correct | Ambiguous | Incorrect | No match | ||||
ITS1 | 32 | 122 | 50.00 | 49.10 | 0.81 | 49.18 | 49.18 | 0.81 | 0.81 | 0.66 |
ITS2 | 32 | 122 | 46.72 | 50.00 | 3.27 | 46.72 | 50.00 | 0.81 | 2.45 | 1.30 |
ITS | 32 | 122 | 58.19 | 39.34 | 2.45 | 55.73 | 38.52 | 1.63 | 4.09 | 0.56 |
trnH-psbA | 25 | 106 | 62.26 | 32.07 | 5.66 | 62.26 | 32.07 | 5.66 | 0.00 | 4.65 |
matK | 28 | 109 | 40.36 | 59.63 | 0.00 | 40.36 | 59.63 | 0.00 | 0.00 | 0.47 |
rbcL | 27 | 113 | 21.23 | 78.76 | 0.00 | 21.23 | 78.76 | 0.00 | 0.00 | 0.13 |
ITS+trnH-psbA | 24 | 99 | 75.75 | 17.17 | 7.07 | 75.75 | 17.17 | 7.07 | 0.00 | 2.51 |
ITS+matK | 24 | 99 | 77.77 | 17.17 | 5.05 | 77.77 | 17.17 | 5.05 | 0.00 | 0.46 |
ITS+rbcL | 27 | 105 | 70.47 | 23.80 | 5.71 | 68.57 | 22.85 | 5.71 | 2.85 | 0.28 |
trnH-psbA+matK | 24 | 101 | 62.37 | 31.68 | 5.94 | 62.37 | 31.68 | 5.94 | 0.00 | 2.27 |
trnH-psbA+rbcL | 25 | 106 | 62.26 | 31.13 | 6.60 | 62.26 | 31.13 | 6.60 | 0.00 | 2.23 |
matK+rbcL | 24 | 101 | 47.52 | 51.48 | 0.99 | 47.52 | 51.48 | 0.99 | 0.00 | 0.33 |
ITS+trnH-psbA+matK | 24 | 99 | 85.85 | 8.08 | 6.06 | 85.85 | 8.08 | 6.06 | 0.00 | 1.70 |
ITS+trnH-psbA+rbcL | 25 | 106 | 77.77 | 13.13 | 9.09 | 77.77 | 13.13 | 9.09 | 0.00 | 1.70 |
ITS+matK+rbcL | 24 | 99 | 80.80 | 12.12 | 7.07 | 80.80 | 12.12 | 7.07 | 0.00 | 0.36 |
trnH-psbA+matK+rbcL | 24 | 101 | 62.37 | 30.69 | 6.93 | 62.37 | 30.69 | 6.93 | 0.00 | 1.58 |
ITS+trnH-psbA+matK+rbcL | 24 | 99 | 86.86 | 5.05 | 8.08 | 86.86 | 5.05 | 8.08 | 0.00 | 1.32 |
* Species represented by multiple individuals.