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. Author manuscript; available in PMC: 2015 May 4.
Published in final edited form as: Dev Dyn. 2014 Jan 4;243(4):601–611. doi: 10.1002/dvdy.24100

Figure 3. At postnatal day 0, Small Leucine Rich Proteoglycans (SLRPs) display differential staining patterns in the endocardial cushion derived structures of the maturing heart.

Figure 3

A–E: Biglycan (green), F–J: decorin (green) K–O: fibromodulin (green) and P–T: lumican (green) immunolocalization in the pulmonary valve (PV), aortic valve (AV), mitral valve (MV) and epicardium. Boxes in A, F, K and P magnified in adjacent panel and designated with a prime. *-annulus region associated with the anterior cusp (AC) of the PV; solid arrows-regions where valve cusps or leaflets anchor into adjacent tissue; solid arrowheads- staining within the cusps or leaflets; open arrowheads- arterial wall staining; open arrows- epicardial staining. PA- pulmonary artery wall; RV-right ventricle; LC-left cusp of the PV; RC-right cusp of the PV; Ao-aortic wall; AL-anterior leaflet of the MV; PL-posterior leaflet of the MV. m- myocardium; Para- paraformaldehyde fixed tissue; Amst- amsterdam fixed tissue. Blue- alpha sarcomeric actin; red- propidium iodide. Scale bars: A= 200um, applies to C, D, F, H, I, K, M, N, P, R, S; A′= 50um, applies to B, E, F′, G, J, K′, L, O, P′, Q, T.