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. Author manuscript; available in PMC: 2015 May 4.
Published in final edited form as: Dev Dyn. 2014 Jan 4;243(4):601–611. doi: 10.1002/dvdy.24100

Figure 5. Immunolocalization of SLRPs in adult murine hearts demonstrated high levels of expression within the cardiac valves and arterial walls of the aortic and pulmonary arteries.

Figure 5

A–C′: Biglycan (green), D–F′: decorin (green), G–I′: fibromodulin (green), J–L′: lumican (green) immunolocalization in the cardiac valves and arterial walls. Boxes in panels B, C, E, F, H, I, K, L magnified in adjacent panels designated with a prime. White outline in B′, E′, H′, and K′ show variability of regions void of SLRP staining. Arrowheads- staining within the cusps and leaflets; solid arrows- cusp/leaflets attachment sites; open arrowheads- staining in the arterial wall; open arrows- staining in the mitral valve leaflets. PV-pulmonary valve; AV-aortic valve; RV- right ventricle; RC-right cusp of the PV; LC- left cusp of the PV; AC-anterior cusp of the PV; NCC- non-coronary cusp of the AV; RCC- right coronary cusp of the AV; LCC- left coronary cusp of the AV; MV- mitral valve; Amst- amsterdam fixed tissue (applies to all panels). Blue- alpha sarcomeric actin; red- propidium iodide. Scale bars: A= 400um, applies to B, D, E, G, H, J, K; B′= 50um, applies to C′, E′, F′, H′, I′, K′, L′; C= 200uL, applies to F, I, L.