Effect of heat stress (HS) and pair-feeding (PF) on (A) heat production per metabolic weight (HP/mBW), (B) net carbohydrate oxidation per metabolic weight (COX/mBW), (C) net fat oxidation per metabolic weight (FOX/mBW), (D) COX/FOX ratio. In experimental period 1 (P1, black) all animals were kept at thermoneutral conditions (TN, THI = 59.7) with ad-libitum feeding (AL) for six days. During period 2 (P2), HS cows (red) were heat-stressed (THI = 76.1), whereas PF cows (blue) were pair-fed in thermoneutrality (THI = 60.0) for six days, once ante partum (ap) and again post-partum (pp). Cows underwent a 24-hours indirect calorimetry analysis on day 5 of each period. Numbers of animals analyzed per group: HSap n = 7, PFap n = 6, HSpp n = 6, PFpp n = 6; all data given as sums of continuous measurements over 24 hours on day 6 of each period; +P < 0.05 in Wilcoxon signed rank sum test for paired samples of the same group; there were no group differences between independent samples of the same reproductive stage and period according to the exact Wilcoxon-Mann-Whitney test (P > 0.05).