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. 2015 May 4;10(5):e0125625. doi: 10.1371/journal.pone.0125625

Fig 5. Fibroblast-derived exosomes prime CSCs to be more chemoresistance.

Fig 5

(A) Transmission electron microscopic image of the exosomes derived from 18Co cells and CAFs. Scale bars, 100nm. (B) Western blotting of CD81 in exosomes. (C) Representative microscopy of SW620 cells exposed to DiI-labeled exosomes for 12h. (D, E) Sphere-forming capacity of SW620 or XhCRC CSCs treated with 18Co/CAF-derived exosomes during chemotherapy (5-Fu or OXA). (F) Sphere-forming capacity of SW620 CSCs treated with ultracentrifugation-purified 18Co-derived exosomes during chemotherapy (5-Fu or OXA). (G, H)Fibroblasts were treated with 10mM GW4869 (dissolves in DMSO) for 24h. The CMs derived from GW4869/DMSO-pretreated fibroblasts were added to CSCs. (I-L) CAF-derived exosomes affected on tumor growth of XhCRC CSCs in female NOD/SCID mice treated with 5-Fu or OXA. Tumor growth curves are shown in I(5-Fu) and K(OXA), and tumor weights and images at the end of experiments are shown in L(5-Fu) and J(OXA). Data are presented as mean ± SD; *P< 0.1; **P< 0.05.