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. 2015 May 4;10(5):e0125625. doi: 10.1371/journal.pone.0125625

Fig 6. Exosomes prime CSCs through mediating Wnt activity.

Fig 6

(A) TOP–GFP levels are associated with CSC maker expression. (B) Sphere-forming efficiency and representative spheres (insert) of TOP-GFP+ and TOP-GFP-/lo SW620 cells. *** P<0.001. (C)Representative flow cytometry and quantification (insert) of TOP-GFP expression of CSCs treated with 18Co-derived exosomes (green line).Gray line represents the control group.(D, E) Representative flow cytometry and quantification (insert) of TOP-GFP expression of CSCs treated with 18Co-derived exosomes (magenta line) or GW4869 (black line)/DMSO (orange line)-pretreated 18Co-CM upon chemotherapy (5-Fu or OXA). Gray line represents the control group. (F) Fibroblast-derived exosomes were purified by ultracentrifugation. The pellet, supernatant and fibroblast cell lysate were subjected to western blotting for exosome maker CD81 and Wnt3a.