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. 2015 May 4;10(5):e0126217. doi: 10.1371/journal.pone.0126217

Fig 4. Removal of HYS-32 restores EB1 comet-like streaks.

Fig 4

Control astrocytes (Con) or astrocytes treated for 24 h with 5 μM HYS-32 (HYS), or treated for 24 h with 5 μM HYS-32 then replaced with normal culture medium for 15 min to 24 h (R15 min, R1 h, or R24 h) were fixed in cold acetone and double-stained for β-tubulin (green) and EB1 (red), and subjected to confocal microscopy. Images were merged to show co-localization (Merged). Square areas were enlarged to show EB1 distribution (Enlarged). Arrowheads indicate microtubule tips. Arrows indicate distribution of EB1 along the microtubules (bars = 5μm).