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. 2015 May 4;10(5):e0126217. doi: 10.1371/journal.pone.0126217

Fig 5. HYS-32 affects the association between EB1 and β-tubulin.

Fig 5

(A) Cell lysates from control astrocytes (Con) or astrocytes treated for 2, 6, 12, or 24 h with 5 μM HYS-32. The cell lysates were immunoprecipitated using normal rabbit serum (NRS) as immunoprecipitation control (IPC) or mouse antibody against β-tubulin (IP: β-tubulin). The immunoprecipitates were then subjected to 10% SDS-PAGE, and analyzed by immunoblotting with antibodies against -tubulin (IB: -tubulin) or EB1 (IB: EB1). (B) Densitometric analyses of EB1 expressed as the density of the bands in the treated groups relative to the control. The results were collected from three independent experiments.*p<0.01 compared to control using one-way ANOVA with Dunnett’s post-hoc test.