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. 2015 May 4;10(5):e0126217. doi: 10.1371/journal.pone.0126217

Fig 6. HYS-32 modulates GSK3β phosphorylation.

Fig 6

(A) Cell lysates from control astrocytes (Con) or astrocytes treated for 2, 6, 12, or 24 h with 5 μM HYS-32 were subjected to 10% SDS-PAGE, and analyzed by immunoblotting with antibodies against GSK3β-pTyr216, GSK3β-pSer9, or GAPDH. (B) Densitometric analyses of GSK3β-pTyr216 and GSK3β-pSer9 expressed as the density of the bands in the treated groups relative to the control. *p<0.05 compared to control using one-way ANOVA with Dunnett’s post-hoc test. The results were collected from five independent experiments. (C) According to the data in (B), the stacked bar graph showing the relative percentage of phospho-GSK3β at indicated time.