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. 2015 May 4;10(5):e0126217. doi: 10.1371/journal.pone.0126217

Fig 7. GSK3β inhibitor SB415286 inhibits the HYS-32-induced phosphorylation of GSK3β-pY216 and attenuates the HYS-32-induced microtubule catastrophes.

Fig 7

(A) Control astrocytes (Con) or astrocytes treated for 24 h with 5 μM HYS-32 (HYS), co-treated for 24 h with 5μM HYS-32and 20μM SB415286 (HYS+SB), or treated with 20 μM SB415286 (SB) were subjected to 10% SDS-PAGE, and analyzed by immunoblotting with antibodies against GSK3β-pTyr216 or GAPDH. (B) Densitometric analyses of GSK3β-pTyr216 expressed as the density of the bands in the treated groups relative to the control. (C) Astrocytes treated as in (A) were fixed in cold acetone and triple-stained for β-tubulin, N-cadherin, and F-actin. Quantitative analysis of the straight distance between microtubule tips and cell border were performed as described in Materials and Methods. The results were collected from three independent experiments. *p<0.01 compared to HYS using one-way ANOVA with Dunnett’s post-hoc test.