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. 2015 May 4;10(5):e0126217. doi: 10.1371/journal.pone.0126217

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© 2015 Chiu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — Control astrocytes (Con) or astrocytes treated for 24 h with 5 μM HYS-32 (HYS), co-treated for 24 h with 5 μM HYS-32 and 20μM SB415286 (HYS+SB) or LY294002 (HYS+LY), or treated with 20 μM SB415286 (SB)or LY294002 (LY) were fixed in cold acetone and double-stained for β-tubulin (green) and EB1 (red) and subjected to confocal microscopy. Images were merged to show co-localization (Merged). Square areas were enlarged to show EB1 distribution (Enlarged). Arrowheads indicate microtubule tips. Double-arrowheads indicate microtubule tips without EB1 signals. Arrows indicate distribution of EB1 along the microtubules. Dashed lines mark the cell border (bars = 5μm).