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. Author manuscript; available in PMC: 2015 May 4.
Published in final edited form as: Gastroenterology. 2008 Sep 6;135(6):1993–2002. doi: 10.1053/j.gastro.2008.08.053

Figure 2.

Figure 2

Enhanced HA binding and rolling flux fraction of CD4+ in TNFΔARE mice. (A and B) CD4+ T cells from the MLN of TNFΔARE mice and WT littermates were incubated with fluorescein isothiocyanate (FITC)-labeled HA (HA-FITC) and analyzed by flow cytometry. Unconjugated soluble HA was used as specificity control (data not shown). Cells were gated on forward scatter, side scatter, and CD4. Representative data are from 4 mice at 20 weeks of age. (C) Physiologic flow conditions were produced using a flow chamber at 2 dyn/cm2. Rolling interactions were analyzed using 107 cells in 8 or more fields of view (pooled mean ± SEM rolling fraction from 3 separate experiments; P < .05).