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. 2015 May 4;10(5):e0125111. doi: 10.1371/journal.pone.0125111

Fig 4. Purinergic modulation of high glucose and palmitate-induced IL-6, IL-8 and COX-2 protein.

Fig 4

HUVECs were exposed to high glucose and palmitate (48 h) in the presence or absence of the P2X7 and P2X4 antagonists. The supernatants were analyzed for IL-6 (A) and IL-8 (B) secretion using ELISA. Cell lysates probed for COX-2 (C; 74 kDa) and normalized to β-Tubulin are represented as percentage of control. A representative immunoblot for each protein is depicted. n = 3 to 4 independent experiments each done in replicates; *p ≤ 0.05.