Fig 8. P2X7 mediates high glucose and palmitate-induced increase in leukocyte adhesion.

HUVEC monolayers seeded in 96-well plates were exposed to high glucose and palmitate in the presence or absence of receptor antagonists for 48 h. Leukocytes labeled with LeukoTracker were allowed to attach for 90 mins after which adherent cells were lysed and the fluorescence was measured at an excitation and emission wavelengths of 480 nm and 520 nm, respectively. n = 4 independent experiments each done in replicates; *p ≤ 0.05.