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. 2015 May 4;10(5):e0126127. doi: 10.1371/journal.pone.0126127

Fig 4. Localization of newly synthesized RNAs to VIBs.

Fig 4

(A) FHM cells were transfected with BrU in treatment with or without ActD and stained with mouse monoclonal antibody against BrU. (B) FHM cells infected with GCRV at MOI of 1 were transfected without BrU (No BrU) in the presence of ActD for 1 h at 9 h post-infection. (C) FHM cells infected with GCRV at MOI of 1 were transfected with BrU (+BrU) in the presence of ActD for 1 h at 9 h or 15 h post-infection. Infected cells were stained with mouse monoclonal antibody against BrU and rabbit polyclonal antibody against NS80. Cell nuclei were counterstained with Hoechst. Scale bars, 10 μm.