Table 1. Oligonucleotides used to produce shRNA expression vectors.
construct | sequences of shRNA (5’-3’) |
---|---|
shRNA-control | F: TGCTGAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT |
R:CCTGAAATGTACTGCGTGGAGACGTCAGTCAGTGGCCAAAACGTCTCCACGCGCAGTACATTTC | |
shRNA-1 | F: TGCTGATCAGAGGAGCACCATATTCGGTTTTGGCCACTGACTGACCGAATATGGCTCCTCTGAT |
R: CCTGATCAGAGGAGCCATATTCGGTCAGTCAGTGGCCAAAACCGAATATGGTGCTCCTCTGATC | |
shRNA-2 | F: TGCTGTTCGAAGTGACGCTCCTTACCGTTTTGGCCACTGACTGACGGTAAGGAGTCACTTCGAA |
R: CCTGTTCGAAGTGACTCCTTACCGTCAGTCAGTGGCCAAAACGGTAAGGAGCGTCACTTCGAAC | |
shRNA-3 | F: TGCTGTGACGTTGAAGAGCGGTGTTGGTTTTGGCCACTGACTGACCAACACCGCTTCAACGTCA |
R: CCTGTGACGTTGAAGCGGTGTTGGTCAGTCAGTGGCCAAAACCAACACCGCTCTTCAACGTCAC | |
shRNA-4 | F: TGCTGTTGGGATTCAAGTTCGACGGCGTTTTGGCCACTGACTGACGCCGTCGATTGAATCCCAA |
R: CCTGTTGGGATTCAATCGACGGCGTCAGTCAGTGGCCAAAACGCCGTCGAACTTGAATCCCAAC |
Uppercase letters indicate oligonucleotides contained 19-mer hairpin structure. Letters in bold show sequence corresponding to S4 genome.