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CaMKIIα activity is enhanced in hippocampal neurons from IRBIT KO mice. (A) Simultaneously imaging of FRET and Ca2+ change (Indo-1) after NMDA stimulation in WT or IRBIT KO cultured hippocampal neurons transfected with mRFP or IRBIT/mRFP. (B) Quantitation of FRET and Ca2+ peak amplitude [Max. −ΔF(Indo-1)] that were expressed as the averaged amplitude of 0–50 s are equal to zero.
