Fig. 6.

Alternative splicing events in thymocytes are enriched for CELF2 target genes. (A) Schematic of T-cell development showing DN and DP stage of thymic development and TCR-α expression and the corresponding changes in CELF2 mRNA expression and inclusion of LEF1 Exon 6 from ref. 16. (B) Table of significant changes in splicing between DN and DP stages of development, as determined by RASL-Seq. Last gene in gray has a P value > 0.05. (C) Validation of CELF2-dependence of genes overlapping in the thymocytes and CELF2-KD RASL experiments in JSL1 cells depleted of CELF2 as in Fig 5C. Percent inclusion values shown are the average of at least three independent experiments. (D) Same as C but including also RNA from FACS purified DN and DP thymocytes. PSI values for thymocytes are the average from at least three independent donors.