Fig. 2.

TXNIP up-regulation following Myc suppression requires MondoA. (A–C) c-Myc levels were decreased by transfection of a c-Myc–specific siRNA pool. Representative experiments performed in triplicate are shown. (A and B) MDA-MB-157 (A) or MDA-MB-231 (B) cells were transfected with either a wild-type TXNIP luciferase reporter construct or a TXNIP promoter construct carrying mutations in the ChoRE MondoA:Mlx binding site. (C) Wild-type MEFs or MondoA-null (Mon^−/−) MEFs were transfected with a wild-type TXNIP luciferase reporter construct. (D) Wild-type or MondoA-null MEFs were treated for 24 h with the indicated concentrations of JQ1, and the levels of the indicated proteins were determined by Western blotting. n, number of independent biological replicates. Values are reported as mean ± SEM.