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. 2015 Apr 6;112(17):E2245–E2252. doi: 10.1073/pnas.1500439112

Fig. 3.

Fig. 3.

Recovery of enzymatic activity of PDIa shows LOC14 reversibly binds to PDIa. PDIa (500 μM) was incubated with either (A) irreversible inhibitor 16F16 (750 μM) or (B) LOC14 (750 μM) for 3 h at room temperature and then diluted 100-fold into assay buffer and analyzed for its ability to inhibit the enzymatic insulin aggregation. Diluted complexes were compared with samples containing 5 μM PDIa only (red) or 5 μM PDIa with either 7.5 or 750 μM compound LOC14 or 16F16. Experiments were performed in triplicate with data plotted as mean ± SEM.