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. 2015 Mar 30;112(17):E2156–E2165. doi: 10.1073/pnas.1501690112

Fig. 1.

Fig. 1.

GhV-G is antigenically distant from Asiatic clade HNV-Gs. Representative polyclonal and monoclonal antibodies made against NiV envelope proteins were tested for cross-reactivity against HeV- and GhV-G. The 293T cells were transfected with C-terminally HA-tagged NiV-, HeV-, and GhV-G expression vectors. pCAGS-transfected cells served as vector controls for nonspecific staining by primary and/or secondary antibodies. At 16 h after transfection, equivalent samples were stained with the serial dilutions of the indicated antibodies and analyzed by FACS. (A and B) Rabbit polyclonal antibodies made by genetic immunization with Nipah viral-like particles (NiVLPs; NiV-M +F +G) and soluble NiV-G (A) or soluble NiV-G alone (B). (C) Mab45 is a well-characterized rabbit monoclonal antibody that recognizes a conserved receptor-binding enhanced (RBE) epitope in NiV- and HeV-G (46). (D) Staining with rabbit polyclonal anti-HA antibodies shows relative surface expression of NiV-G > HeV-G ∼ GhV-G.