Fig. 5.

Fitting of the ab initio model into the desmosome tomography maps. (A) The electron density maps derived from the ET of the human native epidermal desmosome (14) were used to fit the DAMMIN model for Dsg2-Ca. Excellent fit was obtained, as shown in B, where four DAMMIN models illustrate the trans dimer interactions across the midline (MD). (C) Side view of the four DAMMIN models shown in B. Side (D) and top (E) view projections of an idealized array generated with the DAMMIN models from the fitting into the ET maps. A midline (MD) is clearly visible on the side view projection.