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. 2015 Apr 13;112(17):5533–5538. doi: 10.1073/pnas.1504154112

Fig. 5.

Fig. 5.

High level of expression of OGM (PGIP–PG chimera) reduces plant growth and promotes SA accumulation. (A) Levels of OGM and RetOx transcripts in 2-wk-old wild-type (WT) plants, pPR-1:OGM 1 and 2 plants, and in two representative pRetOx:OGM T1 plants. UBQ5 was used as reference. (B) Representative photo of the same plants used in A. (Scale bar, 2 cm.) (C and D) Plants expressing the inducible OGM were germinated in the presence of β-estradiol (C) or were treated with β-estradiol 5 d after germination (D), and fresh weight was measured after 10 d (C) or 5 d (D). Bars: average (n > 10) ± SE; ***P < 0.001, statistical difference between control- and estradiol-treated seedlings (Student’s t test). (E and F) Accumulation of SA in plants expressing the inducible OGM. Four-week-old plants (E) or 10-d-old seedlings (F) were treated with β-estradiol (T) or DMSO (NT) and SA levels were determined after 24 h. Asterisks indicate statistically significant differences with β-estradiol–treated wild-type (WT) plants, according to Student’s t test (P < 0.01).