Table 1.
Co-expression of NK1-r proteins and various nAChR subunit proteins in the neuronal cell bodies of the dorsal thoraco-lumbar spinal cord regions.
| Percent of total Neurons (Neun B positive) that express |
Percent of nAChR positive cell bodies that also express |
||||||
|---|---|---|---|---|---|---|---|
| nAChR | nAChR + NK1-r |
nAChR | nAChR + NK1-r |
Neun B + NK1-r |
Neun B + NK1-r |
||
| nAChR subunits |
Laminae | Control | Sp-SAP | Control | Sp-SAP | ||
| α3 | I-III | 99.4+1.0 | 34.8+2.4 | 96.0+1.0 | 12.6+2.0** | 34.9+2.4 | 13.2+2.0** |
| IV-V | 97.1+1.2 | 30.7+5.9 | 97.8+1.0 | 15.6+2.2* | 31.7+5.9 | 15.9+2.1** | |
| α4 | I-III | 97.2+1.0 | 10.6+1.6 | 96.8+1.2 | 6.6+0.9* | 11.8+2.1 | 6.9+1.3* |
| IV-V | 90.5+2.6 | 9.5+2.4 | 91.4+6.4 | 8.8+1.7 | 10.7+3.0 | 9.7+1.8 | |
| α5 | I-III | 97.9+0.7 | 34.1+5.7 | 93.8+2.5 | 6.7+1.0*** | 34.9+5.9 | 7.3+1.3*** |
| IV-V | 99.8+0.2 | 42.8+7.4 | 99.5+0.5 | 18.2+5.9** | 42.7+7.4 | 18.2+5.9** | |
| β2 | I-III | 90.6+8.3 | 28.9+2.6 | 84.9+13.3 | 9.4+2.7*** | 33.6+5.2 | 12.4+3.0** |
| IV-V | 99.2+0.5 | 33.9+5.3 | 95.9+3.3 | 18.6+4.3** | 34.9+5.3 | 19.7+5.3* | |
| β4 | I-III | 25.1+3.8 | 10.8+1.8 | 24.7+3.1 | 4.5+0.4* | 44.1+2.1 | 19.3+2.4*** |
| IV-V | 19.8+3.0 | 10.2+2.1 | 23.9+3.9 | 5.9+1.2 | 52.6+9.9 | 26.2+4.4** | |
Each value, shown as a mean±sem, represents the percent of neurons that show expression of the specific nAChR subunit, NeuNB (neuronal marker) and NK1 receptor combinations in sP-SAP-treated and vehicle-treated control rats. The treated and control groups were compared by unpaired t-test. Each value represents at least 4 pairs of comparisons. ***, ** and * denote ‘p’ values of <0.001, <0.01 and <0.05 as compared to controls for the same matching column category as in sP-SAP treated rats.